Genetic mapping of the <i>Rcs2</i> locus in soybean cultivar Kent for resistance to frogeye leaf spot

The fungal disease frogeye leaf spot can reduce yield up to 30% in susceptible soybean [Glycine max (L) Merr] cultivars. Rcs2 locus the cultivar Kent provides resistance all named Cercospora sojina races currently found United States, however, this for has not been molecularly mapped yet. F2:3 and recombinant inbred line (RIL) populations derived from a cross of Forrest × were phenotyped greenhouse assays with isolate S23 sojina, reactions determined using visual estimate severity. A bulked segregant analysis linkage mapping Kompetitve Allele Specific PCR (KASP) markers population located on chromosome 11. was RIL same pedigree refined 336 kb interval by evaluating recombination breakpoints RILs. This region contains 31 gene models, polymorphisms 11 these genes and/or promoter regions Williams82.a2.v1 genome. linked reported will be useful marker-assisted selection resistance. research also guide future efforts understand landscape States germplasm discover causal article is protected copyright. All rights reserved